CMIC：微剂量临床试验：尼卡地平在人体血浆中的定量测定及其代谢物的预测（英文版）

A sample treatment procedure and high-sensitive liquid chromatography/tandem mass spectrometry (L.C/MS /MS) method for quantitative determination of nicardipine in human plasma were developedfor a microdose clinical trial with nicardipine, a non-radioisotope labeled drug. The calibration curve waslinear in the range of 1-500 pg/ml, using 1 ml, of plasma. Analytical method validation for the clinical dose.for which the calibration curve was linear in the range of 0.2-100 ng/mL using 20 ul. of